Jurnal

Protective Effects of Catechins Isolate From GMB4 Clone Green Tea Against EPC In Type 2 Diabetes Mellitus

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Introduction : This study aimed to evaluate the role of catechins isolates from GMB4 clone green to in the dynamics of endothelial progenitor cells (EPC) in type 2 diabetes mellitus (DM). Methods: 25 Wistar rats, were divided into 5 groups, including control group, type 2 diabetes mellitus group, treated daily administration of extracts of catechins isolate from GMB4 clones green tea at 20; 40; and 60 mg /kgBB doses for 6 weeks. EPCs analysis was performed by flow cytometry,levels of NO was performed using a spectrophotometer, serum levels of SDF-1a was performed with ELISA technique. Analysis of the expression of SDF-1 and CXCR-4 was performed using immunohistochemistry techniques. Results : The CD34+ and CD133+ count is lower in the DM group compared to the control group (P < 0.05). Of the three doses of catechins, only the highest dose were able to significantly increase CD34+ count compared to rats in the diabetes mellitus group (P < 0.05), Serum NO level is significantly higher in the DM the control group (P < 0.05). All three doses of catechins were able to significantly increase the expression of SDF-1a and CXCR4 in the aorta compared to the DM group or the control group (P < 0.05). Discussion : It can be concluded that high dose cathecin isolate from GMB-4 clone green tea (60 mg/kgBB) may trigger the proliferation and maturation of EPCs in rats with type 2 DM in environment with high level of NO, involving the interacton between SDF-1a and CXCR4 in the aorta.

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Detail Information

Series Title

Jurnal Ners, Vol. 12 No. 2 Oktober 2017

Call Number

(05) 610.5 PRO j

Publisher

Surabaya : Prodi Ilmu Keperawatan FK Unair., 2017

Collation

Hlm. 248-253

Language

English

ISBN/ISSN

1858-3598

Classification

(05) 610.5 PRO j

Content Type

-

Media Type

-

Carrier Type

-

Edition

Vol. 12 No. 2

Subject(s)

Hyperglycemia
aorta
catechins
endothelial progenitor cells

Specific Detail Info

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